Characterizing brain metabolic function ex vivo with acute mouse slice punches
نویسندگان
چکیده
منابع مشابه
Mouse strain differences in metabolic fluxes and function of ex vivo working hearts.
In mice, genetic background is known to influence various parameters, including cardiac function. Its impact on cardiac energy substrate metabolism-a factor known to be closely related to function and contributes to disease development-is, however, unclear. This was examined in this study. In commonly used control mouse substrains SJL/JCrNTac, 129S6/SvEvTac, C57Bl/6J, and C57Bl/6NCrl, we assess...
متن کاملWhole-brain ex-vivo quantitative MRI of the cuprizone mouse
11 Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a sma...
متن کاملSimulated blast overpressure induces specific astrocyte injury in an ex vivo brain slice model
Exposure to explosive blasts can produce functional debilitation in the absence of brain pathology detectable at the scale of current diagnostic imaging. Transient (ms) overpressure components of the primary blast wave are considered to be potentially damaging to the brain. Astrocytes participate in neuronal metabolic maintenance, blood-brain barrier, regulation of homeostatic environment, and ...
متن کاملMechanistic Studies of Antibody-Mediated Clearance of Tau Aggregates Using an ex vivo Brain Slice Model
Recent studies have shown that immunotherapy clears amyloid beta (Aβ) plaques and reduces Aβ levels in mouse models of Alzheimer's disease (AD), as well as in AD patients. Tangle pathology is also relevant for the neurodegeneration in AD, and our studies have shown that active immunization with an AD related phospho-tau peptide reduces aggregated tau within the brain and slows the progression o...
متن کاملSubclassification of brain tumors based on ex vivo MRS metabolic profiles
T. E. Sjøbakk, S. Gulati, M. Gulati, S. Lundgren, S. H. Torp, and I. S. Gribbestad Dept. of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway, St.Olavs University Hospital, Trondheim, Norway, Dept. of Neurosurgery, St. Olavs University Hospital, Trondheim, Norway, Dept. of Oncology, St. Olavs University Hospital, Trondheim, Norway, Dept. of Labor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: STAR Protocols
سال: 2021
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100559